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For RBD production in batch, single colonies were used to inoculate buffered complex glycerol medium (BMGY) medium (1% yeast extract, 2% bactopeptone, 1.34% YNB, 400 g/L biotin, 0.1 M potassium phosphate, pH 6.0, and 1% glycerol) and cultures were grown at 28 C with agitation at 250 rpm until the culture reached a OD 600nm = 46

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Bibcode:2009EnvMM..50..285S

We found that community-dwelling older physically disabled women with metabolically significant vitamin B12 deficiency had a risk of depression that was more than twice as high as that of women without vitamin B12 deficiency