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(Hohhot, Inner Mongolia) with known fertility were randomly selected for the following experiments ( Chemicals Unless otherwise indicated, the chemicals, culture medium, and supplements used in this study were purchased from Sigma-Aldrich (Shanghai, China) and Thermo Fisher (Beijing, China)

At the end of the 6-wk feeding regimen, mice were euthanized by CO 2 asphyxiation

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For wound healing: faster wound closure has been reported within days to weeks in published studies, depending on wound type and severity