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For evaluation of differences in parameters before and after treatment, Wilcoxon signed-rank test or paired Student t-test was used for non-normally and normally distributed variables, respectively

While their mechanisms of action are similar, topical acetyl hexapeptide-8 does demonstrate a lower efficacy than injected Botox, but as researchers note this is to be expected.22 Similar results were found in a small 2013 study, with the total anti-wrinkle efficacy of the Argireline treatment group being 48.9% compared to 0% in the placebo group.23 However, a more recent 2023 study investigating the effects of serum containing Argireline and hyaluronic acid found the results to be not statistically significant.24 This means that more research is needed before claims that Argireline is a true alternative to Botox can be confirmed or denied

Volkow, N.D., Frieden, T.R., Hyde, P.S., & Cha, S.S

Hyperhomocysteinemia due to cystathionine beta synthase deficiency induces dysregulation of genes involved in hepatic lipid homeostasis in mice